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Journal: Cell Reports Medicine
Article Title: Blood metabolic panels for identifying significant fibrosis and inflammation in patients with MASLD
doi: 10.1016/j.xcrm.2025.102522
Figure Lengend Snippet: SA and GAA attenuated MASH progression (A) Schematic workflow. Wild-type (WT) mice were fed an LFD or GAN diet for 20 weeks ( n = 8/group). (B–D) Serum ALT, AST, and TG ( n = 8/group). (E) Representative H&E histology, oil red, and Sirius red staining of liver sections. Scale bar: 100 μm. (F) Liver-to-body weight ratio (Liver index) ( n = 8/group). (G) Hepatic triglycerides ( n = 8/group). (H) NAS ( n = 8/group). (I) Area fraction of oil red O staining (%) ( n = 8/group). (J) Area fraction of Sirius red staining (%) ( n = 8/group). (K) Representative immunofluorescent staining of CD11c and CD206 and immunohistochemical staining of Ly6G in liver sections. Scale bar: 100 μm. (L) Flow cytometry analysis of intrahepatic CD11b + F4/80 + cells ( n = 8/group). (M) Adgre1 (F4/80-coding gene) mRNA expression ( n = 8/group). (N) Ccl2 (MCP1-coding gene) mRNA expression ( n = 8/group). (O) Area fraction of Ly6G staining (%) ( n = 8/group). (P) Ly6g (Ly6G-coding gene) mRNA expression ( n = 8/group). (Q) Tnf (TNF-α-coding gene) mRNA expression ( n = 8/group). (R) Representative immunohistochemical staining of 4-HNE in liver sections. Scale bar: 100 μm. (S) Area fraction of 4-HNE staining (%) ( n = 8/group). (T) GSSG/GSH ratio of mice livers ( n = 8/group). Data are presented as the mean ± SEM. Unpaired Student’s t test was used between two groups. One-way ANOVA was used between multiple groups. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.
Article Snippet:
Techniques: Staining, Immunohistochemical staining, Flow Cytometry, Expressing
Journal: Cell Reports Medicine
Article Title: Blood metabolic panels for identifying significant fibrosis and inflammation in patients with MASLD
doi: 10.1016/j.xcrm.2025.102522
Figure Lengend Snippet: SA and GAA attenuated MASH progression (A) Schematic workflow. Wild-type (WT) mice were fed an LFD or GAN diet for 20 weeks ( n = 8/group). (B–D) Serum ALT, AST, and TG ( n = 8/group). (E) Representative H&E histology, oil red, and Sirius red staining of liver sections. Scale bar: 100 μm. (F) Liver-to-body weight ratio (Liver index) ( n = 8/group). (G) Hepatic triglycerides ( n = 8/group). (H) NAS ( n = 8/group). (I) Area fraction of oil red O staining (%) ( n = 8/group). (J) Area fraction of Sirius red staining (%) ( n = 8/group). (K) Representative immunofluorescent staining of CD11c and CD206 and immunohistochemical staining of Ly6G in liver sections. Scale bar: 100 μm. (L) Flow cytometry analysis of intrahepatic CD11b + F4/80 + cells ( n = 8/group). (M) Adgre1 (F4/80-coding gene) mRNA expression ( n = 8/group). (N) Ccl2 (MCP1-coding gene) mRNA expression ( n = 8/group). (O) Area fraction of Ly6G staining (%) ( n = 8/group). (P) Ly6g (Ly6G-coding gene) mRNA expression ( n = 8/group). (Q) Tnf (TNF-α-coding gene) mRNA expression ( n = 8/group). (R) Representative immunohistochemical staining of 4-HNE in liver sections. Scale bar: 100 μm. (S) Area fraction of 4-HNE staining (%) ( n = 8/group). (T) GSSG/GSH ratio of mice livers ( n = 8/group). Data are presented as the mean ± SEM. Unpaired Student’s t test was used between two groups. One-way ANOVA was used between multiple groups. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.
Article Snippet: Apply fluorescence-labeled secondary antibodies (F4/80 [BioRad, #MCA497], CD206 [BioRad, #MR5D3],
Techniques: Staining, Immunohistochemical staining, Flow Cytometry, Expressing